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Ks-Tek

Spin Column Plasmid Extraction Kit Genome Extraction Column Nucleic Acid Purification Separation Column 2 ml 50 group by ks-Tek

Spin Column Plasmid Extraction Kit Genome Extraction Column Nucleic Acid Purification Separation Column 2 ml 50 group by ks-Tek

$35.36 USD
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Ks-Tek Nucleic Acid Purification Spin Columns
Nucleic Acid Purification Spin Columns are based on proprietary spin column-based silica membrane purification edchnology.In addition to the spin column itself,each spin column comes with one outer tube(reservoir),one frit,one silica membrane and one fixing ring.
 
Under low PH and chaotropic conditions,nucleic acids specifically bind to silica membrane while polysaccharides and proteins pass through.Impurities are further removed by washing.Finally,under low-salt conditions,nucleic acids are desorbed 
and eluted from the membane.

Nucleic acids purified by Nucleic Acid Purification Spin Columns are suitable for various downstream routine applications,Including restriction digestion,PCR amplification,sequencing,ligation and transformation.

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The genome extraction column is composed of an adsorption column and a collection tube. It is suitable for extraction and purification of genomic DNA from a wide range of samples: including tissues, bacteria, cells, plants, blood, viruses, etc., and can quickly prepare up to 20 μg of genomic DNA.
The genomic DNA purified using the above extraction column is suitable for applications such as PCR, enzyme digestion, sequencing, hybridization, and library construction.
Genome extraction column features:

  • Use a wide range of samples.
  • The extracted genomic DNA has large fragments and high purity.
  • High-quality silicone membrane, stable performance, good repeatability.
  • Compatible with mainstream kit recipes.

Genome extraction column specifications:

  • Volume: 2.0 mL
  • Loading volume: 800 μL
  • Purification scale: 0-20μg

​Genome extraction column experiment results:

Collect 1 mL of overnight cultured bacteria and extract genomic DNA with NP20, respectively, with an elution volume of 80 μL.

  • Lane 1: E. coli
  • Lane 2: Staphylococcus aureus
  • Lane 3: Bacillus subtilis

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Picture 1: Electrophoresis of bacterial genomic DNA from different sources

100 μL of human blood, mouse blood and 20 μL of chicken blood and frog blood were processed, and genomic DNA was extracted with NP20, respectively, and the elution volume was 80 μL.

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Picture 2: Extraction rate of blood genomic DNA from different samples

  • Material of gel: Silicon
  • Specification: 2 ml with 4 / 6 / 8 - layers of silicone film
  • Shelf life: 12 months 
  • Material of tube: Plastic

Usage: For sequencing, in vitro transfer Record and translation, restriction enzyme digestion, and conversion of molecular biology experiments.

Reference levels:

  • 4 layer's Gel use for recovery PCR product purification.
  • 6-8 layer's use for  plasmid extraction and genomic DNA extraction
  • Material of tube: Plastic
  • Picture 2: Extraction rate of blood genomic DNA from different samples

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Notice:
1. All centrifugation steps are performed at room temperature unless otherwise indicated.
2. Equilibrate the column before using to activate the silica membrane and increase yield. The column should be used immediately after equilibration.
3. The PH value of supernatant pipetted into spin column should be below 7.0
4. The concentration of ethanol should be within 55%-80%.
5. Elution can be used by Elution Buffer or ddH2O. For long-term storage of DNA, Elution
Buffer is recommended.
6. When elution is used by ddH2O,the PH value should be controlled within 7.5-8.0.

  • The product is accompanied by a complimentary detailed instruction

小提柱说明书22in the box.
​ We produce membrane filters and syringe filter and more laboratory consumables.

*****  A world class manufacturing facility, Class 10,000 cleanroom;
*****  ISO 9001, ISO 13485 certificated, CE certificated;
*****  Products quality achieved by Japanese quality control system during production;
*****  Cost-effective, state-of-the-art production technology.

 

 

 

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